An experiment involving 40 adult trotters and saddle horses Pressure Prevention Mattresses was conducted during a period of one year in order to investigate the influence of vitamin A (retinol) and D (25-(OH)D) supplementation on serum vitamin concentrations and the seasonal variation of the serum concentrations of these vitamins.Vitamin supplementation was started either at the beginning or in the middle of the indoor (winter) feeding period.Supplementation lasted from the beginning of September or January to the end of May.According to the results, neither the dietary vitamin supplementation nor the length of the grazing period had any consistent effect on the serum vitamin concentrations.Neither was there any systematic seasonal variation in the serum tack copyright retinol and 25-hydroxyvitamin D levels.